Authors: T. López, E. Ortiz-Islas, P. Guevara, E. Gómez, H. Monroy, O. Novaro
Affilation: National Institute of Neurology and Neurosurgery, Mexico
Pages: 371 - 374
Keywords: brain cancer, copper complexes, drug delivery, binary oxide
In the present work, we are reporting the toxic effect to brain cancer cells exerted by copper complexes loaded (CC) on a binary oxide. For this purpose, the TiO2-SiO2 oxide was prepared using the sol-gel technology. Individually, copper acetylacetonate and ammonium tetrachlorocuprate were incorporated to the oxide during the synthesis process. Copper complexes and CC/TiO2-SiO2 were characterized by different physical-chemical techniques. The cytotoxic properties of both pure complexes as these loaded on TiO2-SiO2 were determined using the rat glioblastoma C6 cell line. The FTIR and UV-VIS spectroscopy indicate that copper complexes were success loaded on the oxide without changes in their structures. Release profiles shown two steps of copper complexes released. C6 cells were exposed to copper complexes and CC-TiO2-SiO2 materials at different concentrations and 24 hours post treatment the cell viability was tested by MTT assay. Cell viability assay showed a high cytotoxic effect of the complexes alone and them released from the SiO2-TiO2 on the cancer cells; however, both showed better toxic effect than Cis-Pt. SiO2-TiO2 alone had a minor cytotoxicity which indicates its high biocompatibility with these cells. There was a mitochondrial fragmentation when COS-7 cells were treated with the Cu/TiO2-SiO2 material during 12 hours.