Authors: H. Lee, S. Row, L. Xu, S.T. Andreadis, K.W. Oh
Affilation: SUNY at Buffalo, United States
Pages: 145 - 148
Keywords: microwell array, PDMS
We describe a simple micro-fabrication method by using polydimethylsiloxane (PDMS) patterns on a glass substrate that enables simultaneous control of cell-to-cell adhesion. While the microwells used previously facilitated the cell-to-cell formation by PDMS stamping method, not only should it be carefully controlled to avoid nonuniform patterns by deformation, but it is difficult to create thick patterns that allow the cells to be trapped into the microwells. To overcome this technical challenge, a simple and robust fabrication method is demonstrated to study cell-to-cell interaction of Human Bone Marrow Mesenchymal Stem Cells (BM-MSCs). In addition, we investigate pattern transfer success rate from the PDMS pattern to the glass substrate by varying the surface area of patterns contacted on the glass.