Authors: M.S. Bae, S.E. Kim, J.S. Na, I.K. Kwon
Affilation: School of Dentistry, Kyung Hee University, Korea
Pages: 354 - 357
Keywords: hyaluronic acid, hydrogel, simvastatin acid, bone regeneration
In the field of bone tissue engineering, various biomaterials have been developed in conjugation with bone inducing factors for bone tissue regeneration due to their distinct activities of osteoconductivity and osteoinductivity. In this study, we have developed photocurable hyaluronic acid (HA) based hydrogels for bone regeneration, and loaded simvastatin acid, which known to be effective on osteogenic differentiation, in hydrogels as a bone inducing factor in order to accelerate bone ingrowth. The mechanical property and swelling ratio of photocurable hydrogel and simvastatin acid release activity was controlled by crosslinking degree with 2-aminoethyl methacrylate and the initial loading amount of simvastatin acid, and the cytotoxicity and osteogenic inducing activity was evaluated. Finally, the in vivo bone forming activity was evaluated by implanting the developed biomaterial in animal. On soft X-ray radiography and CBCT (Cone beam computed tomography), in the experimental group, those of which were terminated at 3, 5, 7 and 9 weeks after the formation of defective bones, showed the extended formation of trabeculation in the center and peripheral regions of the defective bone in comparison with in the controlled group. This study showed potent application of a hybridized biomaterial in tissue engineering, particularly in the regeneration of bone.