Nanotech 2010 Vol. 3
Nanotech 2010 Vol. 3
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy

Bio Sensors, Diagnostics & Imaging Chapter 1

Label-free, Fluorescenece Detection of DNA Nanoparticle Scaffolds

Authors: A.J. Kell, S. Tan, I. Charlebois, M. Boissinot, M. LeClerc, B. Simard

Affilation: National Research Council, Canada

Pages: 1 - 3

Keywords: DNA, label-free detection, nanoparticle, polymeric transduction

We have developed a nanoparticle-based DNA biosensor that can detect unlabelled ssDNA with good sensitivity (picomolar concentrations). The biosensor makes use of the optical changes of a cationic polymer interacting with ssDNA versus that of hybridized DNA to provide a fluorescence signal only when a specific hybridization event occurs (i.e. only when perfectly complementary DNA is present)

ISBN: 978-1-4398-3415-2
Pages: 880
Hardcopy: $189.95