Nanotech 2009 Vol. 2
Nanotech 2009 Vol. 2
Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites

Environment, Water, Health, Safety and Society Chapter 7

Cytotoxicity and genotoxicity of silver nanomaterials

Authors: B. Uyguy, M.D. Mason, A-K. Ng

Affilation: University of Southern Maine, United States

Pages: 383 - 386

Keywords: silver nanomaterials, cytotoxicity, genotoxicity

Summary We have investigated the cytotoxic effect of dialyzed silver nanoparticles (Ag NPs), PEGylated Ag NPs and silver nanorods on human cultured cells. The cytotoxicity of 24 h treatment with Ag NPs at 0.25, 0.5, 1.0, 5 and 10 ug/cm2 were as follows: 97.5%, 91.7 %, 81.8 %, 27.8% and 24.7% relative survival, respectively, for umbilical vein endothelial cells HUVEC; 99%, 95.6%, 92%, 0.11 % and 0% relative survival, respectively, for T leukemia cell line JURKAT; and 97.3%, 96%, 87.5%, 65% and 51.8% relative survival, respectively, for human PBMC. The Ag NPs showed higher toxic effect at longer exposure time, 120 h: 63.7%, 57.63 %, 34.23 %, 1.03 % and 0 % relative survival were found in HUVEC treated with Ag NPs at 0.250, 0.5, 1.0, 5 and 10 ug/cm2 ,respectively. Such treatment resulted in no survival for JURKAT and B lymphoblastoid cell line RAJI. PEGylated Ag NPs appeared to be less toxic to JURKAT. Among the 3 types of silver nanomaterials, silver nanorods were found to have higher toxic effect on the human cell lines tested. Using the cytokinesis-block micronucleus assay we also demonstrate that Ag NPs can cause DNA damage in human peripheral blood cells. Topic area: Toxicology

ISBN: 978-1-4398-1783-4
Pages: 590
Hardcopy: $179.95

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