Nanotech 2009 Vol. 2
Nanotech 2009 Vol. 2
Nanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites

Nano Bio Materials and Tissues Chapter 6

Immobilization of Enzymes on Functionalized Magnetic Nanoparticles for Efficient Biocatalysis

Authors: W. Wang, Z. Li, D.I.C. Wang

Affilation: National University of Singapore, Singapore

Pages: 337 - 339

Keywords: magnetic nanoparticles, immobilization of enzymes, core-shell, superparamagnetism, biocatalysis

In this paper, iron oxide magnetic nanoparticles were synthesized by co-precipitation method and were functionalized with aldehyde group. Spherical superparamagnetic nanoparticles with diameter of 100 nm were obtained based on the results of field emission scanning electron microscopy (FESEM) and vibrating sample magnetometer (VSM). These particles were further applied for immobilization of enzymes by covalent binding between aldehyde group on particles and amino group on the enzyme surface. Different pH was applied for optimization of the immobilization conditions. The result showed that pH 4.0 is the best pH for immobilizing BSA on the nanometric support.

ISBN: 978-1-4398-1783-4
Pages: 590
Hardcopy: $179.95