Authors: N. Goel, R. Sharma
Affilation: Pt BDS Post Graduate Institute of Medical Sciences, India
Pages: 229 - 232
Keywords: infant HIV, DNA, RNA, CD4/CD8 counting, nanobarcode
Background: New methods have emerged in detection and monitoring HIV using nanotechnology techniques but HIV in infants got less attention. Purpose: We report here the Human Immunodeficiency virus (HIV) and Acquired Immune Deficiency Syndrome (AIDS) of under 5 years old children, especially in developing countries. Study Design: Different from adult HIV, in infants it is a challenge of accuracy in diagnosing HIV infection. The success depends on accuracy and sensitivity of HIV infection diagnostics. Materials and Methods: Different HIV-1 DNA and RNA Assays, P24 antigen assay, CD4 counting and DBS methods were compared for infant HIV diagnosis and feasibility in tertiary care hospital set up. Results: The HIV DNA and RNA assays were qualitative and semiquantitative methods. The P24 antigen and CD4/CD8 counting were new quantitative possibility of nanotechnology based bio sensors for anti-HIV antibody detection in saliva. Other methods p24 antigen biobarcode amplification, nanoarray and FITC(Fluorescein-5-isothiocyanate) doped silica nanoparticle for CD4/CD8 counting were upcoming possible biomarkers but these are in infancy. Conclusion: HIV DNA and RNA techniques offer good HIV detection in infants. P24 antigen and CD4/CD8 counting offer better quantitative results and further precision by nanoparticle based miniature bar code analysis.
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