Biosensors and Diagnostics – TechConnect Briefs

Multiple drugs of abuse test for saliva specimen on a single lab-on-a-film-chip using electrochemical immunoassay

Kim Y.H., Park J.H., Nam H.H., Choi M.H., Jung S.H., Song G.J., Nano-Ditech Corporation, US

A novel diagnostic electrochemical immunoassay device integrating lab-on-a-film-chip (LOFC) and microfluidic technologies with chromatographic methods of rapid immunoassays for In Vitro Diagnostics (IVD) has been developed. This LOFC device is produced by a lithography technique [...]

Ultrasensitive Protein Detection Using an Au/ZnO SPR-Based Biosensor

Chang C-C, Lin C-W, Lin S-H, Chiu N.-F., Lin C-W, Lin S-H, National Taiwan University, TW

In this study, we demonstrated that the Au/ZnO nanocomposite films could be effectively used to enhance the performance of SPR for the detection of tumor marker. Carbohydrate antigen 15.3 (CA15.3) tumor marker for breast cancer [...]

Understanding the formation and adsorption of 2-dimensional and 3-dimensional protein aggregates on a solid surface

Rabe M., Verdes D., Seeger S., University of Zurich, CH

We have comprehensively investigated the structure of protein assemblies that form spontaneously upon protein adsorption on solid interfaces using a surface sensitive fluorescence imaging technique. It was found that proteins usually do not cover surfaces [...]

Identification of bacterial drug resistance in blood

Kaittanis C., Nath S., Perez J.M., University of Central Florida, US

Development of antibiotic resistance by microorganisms has been on the rise in recent years. Therefore, identifying drug resistant strains and effective antibiotic agents is critical. Traditional microbiological methods assess antimicrobial susceptibility within 24 to 48 [...]

Precise positioning of particles in microwells within horizontal DEP cages

Lombardini M., Bocchi M., Giulianelli L., Guerrieri R., ARCES, IT

The analysis and manipulation of one or few biological cells or artificial particles in the micron size range is a fundamental requirement in various life sciences applications such as sorting of rare cells or high-efficient [...]

Force Discrimination for Enhanced Biosensor Specificity of Micro-Retroreflector Assays

Knoop J.D., Kemper S.M., Sherlock T., Cacao E., Ruchhoeft P., Willson R.C., University of Houston, US

In nearly all diagnostic/bioanalytical methods other than mass spectrometry, what is actually detected is a label of some sort, and the sensitivity and simplicity of detection of this label limits assay performance. Non-specific interactions between [...]

Development of a Flow-cell System to Quantify Analyte-receptor Binding Kinetics Utilizing Guided-mode Resonance Sensors

Wawro D., Zimmerman S., Ding Y., Magnusson R., Moss E., Michalik J., Resonant Sensors Incorporated, US

A novel flow-cell system integrated with a guided-mode resonance sensor element array is presented. The association/disassociation binding cycle is monitored for protein-protein and protein-cell interactions. The real-time binding interactions of the antibody IgG and protein-A [...]

Carbon Nanotube Array Sensor for MEMS Lab-on-a-chip

Joseph L.B., Veedu V., Garmire D., Oceanit and University of Hawaii, US

Lab-on-a-chip devices have the potential to provide point-of-care diagnostics that can save many thousands of lives per year. One of the primary limitations to wide-spread adoption of lab-on-a-chips is the excessive cost of fabrication and [...]

Application of QCM DNA biosensor to detect a marine derived pathogenic virus VHSV

Hong S-R., Hong S., Hong S-R., Hong S., Kangnung National University, KR

Each year infectious diseases cause substantial economic losses for public and commercial aquaculture ventures. VHS is one of most serious viral diseases caused by VHSV and damaging both fresh and marine fish species. Detecting VHSV [...]

CdSe nano-particles coated with thiol-terminated oligo(ethylene oxide)for protein targeting reagent

Ito Y., Tsutsumi H., Yamaguchi University, JP

Fluorescent labeling reagents are used as medical and biochemical research reagents that label specific protein, antigen, antibody, and DNA. However, the chromophoric groups (organic compounds) usually are not stable under long-time ultra violet (UV) excitation. [...]

Portable FRET Sensing of Proteins, Hormones, and Toxins Using DNA Aptamers and Quantum Dots

Jackson G.W., Strych U., Frank E., Willson R.C., Ballerstadt R., McNichols R.J., BioTex, Inc., US

There is an urgent need to develop methods and devices that make the monitoring of any compound of interest in the public water system more reliable, more sensitive and more economical. We are developing a [...]

Biochip for minimally invasive blood glucose monitoring

Zhang P., Ahmadi M.M., Jullien G.A., University of Calgary, CA

The most common techniques for blood glucose sensing are based on enzymatic glucose oxidation techniques because of being highly sensitive and specific to the glucose. But, the techniques need oxygen environment and the measurement accuracy [...]

Characterization and Applications of Modified Gold Electrode Sensor with Nanoparticles

Leung S.W., Wang Y., Gu H., Lai J.C.K., Idaho State University, US

In the recent development of biosensor, response time of the sensor is drastically improved by using direct coupling of enzymes onto the surface of electrode. The drawback of the direct coupling of enzymes is denature [...]

A 3-dimensional nanobiosensor based on an anodic aluminum oxide template

Wang G.-J., Lin Y.-D., National Chung Hsing University, TW

In this paper, we propose a high sensitive nanobiosensor based on a 3D sensing element that has uniformly deposited gold nanoparticles. The sequential synthesis processes include: preparation of an AAO film; modification of the surface [...]

Fiber Optic Biosensors Detecting System of Laser Processing Micro Channels

Bai Z-C, Qin S-J, Zhang X., Guizhou University, CN

With the development of micromachining technology, smart micro sensors have been a very potential technology. Micro channels products fabricated by micromachining technology have been put into markets in last years, such as inkjet tube of [...]

Real-time single-molecule dynamics observed with a nanoscale transistor

Brousseau L.C., Quantum Logic Devices, US

Direct observation of single molecular reactions and motions are impossible by optical techniques due to diffraction limits. However, ensemble measurements of binding dynamics in biological systems offer limited understanding of individual actors in metabolic and [...]

Infant HIV and Nanotechnology

Goel N., Sharma R., Pt BDS Post Graduate Institute of Medical Sciences, IN

Background: New methods have emerged in detection and monitoring HIV using nanotechnology techniques but HIV in infants got less attention. Purpose: We report here the Human Immunodeficiency virus (HIV) and Acquired Immune Deficiency Syndrome (AIDS) [...]

Performance Comparisons of Nanoparticle Modified Sensor Electrodes for the Detection of Nitrite and Peroxide

Lai J.C.K., Wang Y., Gao W., Gu H., Leung S.W., Idaho State University, US

For the third generation of biosensor that electrode is coupled directly with enzymes, the response time for detection is rapid but stability of the measurements and the coupled enzymes is a concern. In our research [...]

Label-free direct detection of analytes from optically inaccessible samples

Brousseau L.C., Quantum Logic Devices, US

Fluorescent optical methods for biomolecular detection are widely used for research and diagnostic applications. However, these techniques are useful only in sample matrices that are optically transparent non-scattering, which precludes most biologically relevant fluids, such [...]

Extended Applications of Picomolar Technology to Measure Immunoactive Biomarkers

Shriniwas B.D., Sharma A., Sharma R., Sharma A., Sharma R., RTM Nagpur University, IN

The picotechnology extended use in marine and biology applications are reported after our report on picotechnology to measure picomolar quantities of single stranded DNA in evaluation of Texotere chemosensitivity (TCRT,2008,28(6):175). Lead and mercury poisoning is [...]

Relaxation Time Constants of Nanometals in Human Tissues and Clinical Applications

Sharma A., Sharma R., Sharma A., Sharma R., Center of Nanotechnology, IN

The metals are in abundance in human body in biofluids, blood, cerebrospinal fluid, saliva, lymph. These metals are found in free and bound forms in each tissue and their amount is tissue specific. The mechanism [...]

Monitoring of the major blood vessels supplying head and brain (carotid arteries) contactlessly

Kaminska B., Simon Fraser University, CA

An apparatus for measuring Carotid Arteries movement without physically connecting electrodes or other sensors to the body was developed. The measurement system was implemented to simultaneously monitor the Carotid Arteries and ECG in order to [...]

Fluorescence Based Specific PNA-DNA Binding Characterization on nano-FET DNA Device Surface

Rastogi S.K., Mishra N.N., Finaloski B., Cameron E., Maki W., Postdoc Researcher, US

A novel sequence of peptide nucleic acid (PNA; beta-Ala-(T10)-beta-Ala-NH2) containing ten thymine monomers and beta-alanine amino acid at both (C and N) terminal of main chain was synthesized manually using solid phase tBoc peptide chemistry. [...]

Photothermal Characteristics study of PEGylated Gold Nanorods

Shih T-Y, Lin J-T., Tien C.C., Tseng P-L, Chen Y-H, Industrial Technology Research Institute, TW

Gold nanorods have a growing role in a diversity of medical applications especially in the area of hyperthermal therapy of tumor. Since localized temperature rising by laser irritation gold nanorods at near-IR frequency can induce [...]

SERS Gold Nanoparticles for Cancer Surface Marker Detection

Nguyen C.T., Nguyen J., Nguyen C.T., Nguyen J., Rutledge S., Wang C., Walker G.C., University of Toronto, CA

Surface Enhanced Raman Scattering (SERS) gold nanoparticles may provide novel optical tags for cellular analysis. Currently, cell markers are mostly assessed by fluorescence labeling using flow cytometry. However, fluorescence dyes are usually limited to 4-5 [...]

Magnetic Nano Particles for Diagnostics

Nguyen K.C., Pham V.H., Do S.T., Tieu D.T., SAIGON HI TECH PARK, VN

Magnetic Nano Particles (MNP) for diagnostics had been fabricated by coating the particle ‘s surface with specific coupling agents . This agent allows the pulling the virus containing DNA and/or RNA molecules off the patient [...]

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