Authors: D. Bianchi, D. Serway, W. Tamashiro
Affilation: Spectrum Laboratories, United States
Pages: 359 - 362
Keywords: purification, hollow fiber filters, diafiltration, tangential flow filtration, scale-up
Hollow Fiber Diafiltration (or Hollow Fiber Tangential Flow Filtration) is an efficient and rapid alternative to traditional methods of nanoparticle purification such as ultracentrifugation, stirred cell filtration, dialysis, or chromatography. Hollow Fiber Diafiltration can be used to purify a wide range of nanoparticles including liposomes, colloids, magnetic particles and nanotubes. Hollow Fiber Diafiltration is a membrane based method where pore size determines the retention or transmission of solution components. It is a continuous flow process where the sample is gently circulated through a tubular membrane. With the controlled replacement of the permeate (or dialysate), highly pure nanoparticle solutions can be attained. Unlike conventional dialysis, which operates by a slow diffusion mechanism, hollow fiber diafiltration is a faster pump-driven method. Direct scalablity from R&D volumes through full production is another advantage of hollow fiber diafiltration. Simply by adding more membrane fibers and maintaining a few operating parameters, large volumes can be processed in the same time and under identical pressures and flow dynamics as bench-scale volumes. Continuous flow, precise membrane pore size and a module design scalable to high membrane areas provide a rapid, high-purity and fully scalable nanoparticle diafiltration process.