Authors: Z-C Bai, S-J Qin
Affilation: Guizhou University, China
Pages: 331 - 334
Keywords: nano crystalline, bio-sensor, microchannels, fluorescent detection
Single molecule detection has been a hot point problem of science and research, classic dye fluorescent marking technology has a many defects, for example fluorescent power low, fluorescent instability , and so on. Fluorescent quantum dot of nano crystalline cluster has many merit for a material marked with fluorescence, such as size small, special chemistry characterization. shell-core structure of ZnSe nano crystalline cluster is used for marking glucose molecules in this paper; fluorescent detection is successfully realized in microchannels(fluorescent and absorb figure of ZnSe nano crystalline cluster is in figure 1 and figure 2) . mercaptoacetic acid is used for packing ZnSe fluorescent quantum dot, which is dissolved in the water. carboxide of mercaptoacetic acid is used for connecting glucose molecule, and mixture solution of glucose molecule marked with ZnSe nano crystalline cluster is inleted with capillary force in multi-microchannels which diameter is 30 microns . exciting light in the range of wavelength from 265nm to 420nm is used for exciting glucose molecules marked with ZnSe nano crystalline cluster in multi-microchannels, and fluorescent detection is realized for glucose molecules. It is concluded that shell-core structure of ZnSe nano crystalline cluster is feasible for marking glucose molecules, the result is indicated in figure 3 and figure 4. The new method based on this study will lead to new application in many fields of biologic analysis, bio-sensor, biologic detection, voyage and spaceflight, and so on.
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