Authors: J. Seo, J. Jung, S-I. Han, K-H Han, Y. Jung, A. Bruno Frazier
Affilation: Inje University, Korea
Pages: 320 - 323
Keywords: blood cell, cell, dielectrophoresis, lateral-driven, microfluidic
This paper presents lateral-driven continuous dielectrophoretic (DEP) microseparator for separating red and white blood cells suspended in a highly conductive dilute whole blood. The DEP microseparators enable the separation of blood cells with continuous operation based on the lateral DEP force without the use of additives such as tagging or probing materials. Experimental results show that divergent type DEP microseparator can continuously separate out 87.0% of the red blood cells (RBCs) and 92.1% of the white blood cells (WBCs) from diluted whole blood, while convergent type can separate 93.6% of the RBCs and 76.9% of the WBCs, within 5 minutes simply by using a 2-MHz 3-Vp-p AC voltage to create a gradient electric field in suspension medium with a high conductivity of 17 mS/cm.