Nanotech 2008 Vol. 2
Nanotech 2008 Vol. 2
Nanotechnology 2008: Life Sciences, Medicine & Bio Materials - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2

Drug & Gene Delivery Systems Chapter 4

SiRNA for Therapeutic Immuno-modulation: Simultaneous Delivery of Cytokine-targeted SiRNA and DNA Antigens to Dendritic Cells using Polymer Microcarriers

Authors: A. Singh, H. Nie, B. Ghosn, K. Roy

Affilation: The University of Texas at Austin, United States

Pages: 350 - 353

Keywords: immunotherapy, cytokine, dendritic cells, vaccine, IL-10

RNA interference mediated gene knockdown is a potential immuno-modulatory tool for tuning dendritic cells (DC) activation and function in-vivo. We have developed a novel multi-tiered delivery system for combinatorial administration of SiRNA and plasmid DNA antigens in a single gene carrier system. Our hypothesis is that, co-delivery of IL-10 specific SiRNA along with DNA antigens to the same dendritic cells would inhibit IL-10 production, enhance DC activation, antigen presentation and T cell response. Non-toxic, polyethyleneimine (PEI)-functionalized, poly(lactic-co-glycolic acid) (PEI-PLGA) microparticles co-delivering IL-10 SiRNA and DNA antigen exhibited significantly higher (p

ISBN: 978-1-4200-8504-4
Pages: 808
Hardcopy: $159.95