Nanotech 2007 Vol. 3
Nanotech 2007 Vol. 3
Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 3

Micro and Nano Fluidics Chapter 4

A method for the highly parallel analysis of gene expression of single cells

Authors: W.R.A. Meuleman, N. Milner, D.W.K. Lueerssen and E.M. Southern

Affilation: Oxford Gene Technology, United Kingdom

Pages: 296 - 299

Keywords: DNA microarrays, hybridisation, single cell

Presented is a method for carrying out rapid single cell gene expression analyses on a large number of cells in parallel. The entire analysis is carried out on a DNA microarray. We demonstrate single molecule resolution without the use of PCR, allowing us to quantitatively analyse high as well as low levels of gene expression. Because of the parallel nature of the method, individual cells within a population can be compared rapidly and conveniently.

ISBN: 1-4200-6184-4
Pages: 732
Hardcopy: $139.95