Nanotech 2006 Vol. 2
Nanotech 2006 Vol. 2
Technical Proceedings of the 2006 NSTI Nanotechnology Conference and Trade Show, Volume 2

Bio Sensors and Diagnostics Chapter 3

A Sensitive Peptide-Nanotube Based Pathogen Assay

Authors: R.I. MacCuspie, I.A. Banerjee, S. Gummalla, H. Mostowski, P. Krause and H. Matsui

Affilation: Hunter College, United States

Pages: 264 - 266

Keywords: biosensor, peptide, nanotube, flow cytometry

A novel peptide nanotube-based multiplexed pathogen assay has been developed that has high sensitivity, detecting as few as 100 pathogens per mL in as little as 0.5mL. Multifunctional nanotubes detect viruses by selectively binding antibody at the ends of the nanotube to the virus, while the length of the sidewall is used for signaling with fluorescent dyes. Multiple nanotubes bind one virus at low pathogen levels and begin to form three dimensional networks of many nanotubes binding many pathogens as observed by TEM. Discussion will include details on this mechanism of network formation. These single and multiple virus network structures are quantified by flow cytometry. By assigning unique fluorescent dyes to each antibody used, one can create a multiplex assay limited only by the ability to resolve each dye. Proof of principle experiments with several viruses, including Herpes Simplex Virus Type 2, Adenovirus Type 3 and Type 5 and Influenza strains A and B will be presented.

ISBN: 0-9767985-7-3
Pages: 893
Hardcopy: $119.95