Nanotech 2006 Vol. 2
Nanotech 2006 Vol. 2
Technical Proceedings of the 2006 NSTI Nanotechnology Conference and Trade Show, Volume 2

Bio Sensors and Diagnostics Chapter 3

Surface modification and characterization of a cyclic olefin copolymer for magnetic bead-based stop-flow microfluidic ELISA

Authors: M. Herrmann, T. Veres and M. Tabrizian

Affilation: McGill University, Canada

Pages: 244 - 247

Keywords: bio sensor, lab-on-chip, microfluidic, fluorescence, magnetic beads, immunoassay

This abstract describes a microfluidic platform for simple, highly effective and parallel Enzyme Linked Immuno-Sorbent Assay (ELISA). The method is based on the use of magnetic micro-beads in order to increase the capture rate of the analyte in stop-flow conditions and create an internal mixing. The enzymatic amplification of the fluorescent signal contributes to increase the sensitivity of protein detection in an immunoassay similar to conventional ELISA. Additionally, the design allows readily performing parallel measurements.

ISBN: 0-9767985-7-3
Pages: 893
Hardcopy: $119.95