Papers:
Nanobiodevices Integrating Biomolecular Motors
Biomolecular motors, such as the motor protein kinesin, can serve as force-generating modules in hybrid nanodevices. We will present recent progress in utilizing these motors for nanoscale transport of target reagents and discuss applications, such [...]
Surface Characterization of a Direct, Label-Free Piezoelectric Immunosensor Platform
Papadopoulou-Bouraoui A., Lejeune M., Barrero-Moreno J., Valsesia A., Manso M., Gilliland D., Ceccone G., Rossi F., European Commission - JRC, IT
During this study the QCM (Quartz Crystal Microbalance) crystal surface was functionalized in order to provide active amino groups, and serve as an active platform for antibody immobilization and the development of direct, label-free immunosensors. [...]
New Light Induced Molecular Switch allows Sterically Oriented Micrometer Sized Immobilization of Biomolecules
Snabe T., Neves-Petersen M.T., Fojan P., Klitgaard S., Neves-Petersen M.T., Petersen S.B., Aalborg University, DK
Fundamental knowledge on protein structures and the effect of ultraviolet light on these structures has paved the way for the development of a unique light-based immobilization technology that allows oriented protein immobilization onto micrometer sized [...]
Nano-Cantilevers for an Ultra-Sensitive Bio-Assay
Zheng J., Klein K., Pucha R., Sitaraman S.K., Sitaraman S.V., Merlin D., Rajalakshmi S., Sarma D.S.R., Gerwirtz A., Sitaraman S.K., Sitaraman S.V., Georgia Institute of Technology, US
Micro and Nano-scale cantilever arrays are being developed for an ultra sensitive bio-assay (Figure 1). The advantage of these cantilevers is that they eliminate the use of laser for deflection detection, which is impractical for [...]
Characterization of a Tethering System for Biosensor Applications
Blasi L., Pompa P.P., Pisignano D., Palazzo G., Mallardi A., Maruccio G., Maffei A., Ciccarella G., Vasapollo G., Calabi F., Cingolani R., Rinaldi R., Leece University, IT
The immobilization of brain dopamine receptors on a solid support can represent an appealing strategy for the realisation of a sensing element for dopamine and dopaminergic drugs. Brain dopamine receptors are the primary targets in [...]
Plasmon-enhanced Fluorescence Detection of Blood Metabolites in Biosensors
Klitgaard S., Rasmussen T., Neves-Petersen M.T., Neves-Petersen M.T., Petersen S.B., Aalborg University, DK
Quick and precise detection of blood metabolites is a key parameter in monitoring and treating critically sick patients. Each analysis requires a certain amount of blood, and especially for babies in neonatal care repeated sampling [...]
Synthesis of new Metal Complex – Biomolecule Hybrids as Redox Sensors for Specific Nucleobases
There are a number of redox-active supramolecular receptors described in the literature, but those containing ferrocene as a redox-active reporter group are the most frequently cited. It has been shown that modified ferrocenyl conjugates can [...]
Signal Amplification of Surface Plasmon Resonance based on Gold Nanoparticle-Antibody Conjugate and its Application to Protein Array
Nanoparticles have offered many diverse capabilities in bioanalysis and biotechnological applications, due to the intrinsic properties of nanoparticle that is distinguished from bulk materials. The integration of nanoparticle, which exhibit unique electronic, photonic, and catalytic [...]
Microcantilever Array Sensor for DNA and RNA Detection
Cherian S., Natesan M., Macis E., Cain R., Tedeschi L., Citti L., Palchetti I., Tombelli S., Raiteri R., University of Genova, IT
A microcantilever array platform is used for the detection of DNA hybridization and mRNA using selected immobilized oligonucleotides. Parallel detection on sixteen cantilevers are carried out simultaneously. The immobilization procedure and the results of the [...]
The Use of Quartz Crystal Microbalance in Cell Differentiation Detection
Shih C-J, Ke W-W, Lieu C-H, Industrial Technology Research Institute/Electronics Research & Service Organization, TW
We try to investigate whether the hematopoietic cell differentiation can be detected by Quartz Crystal Microbalance. Because certain hematopoietic transcription factors play a critical role in cell differentiation and lineage determination, they can be alternate [...]
Multiwalled Carbon Nanotubes as Platforms for the Design of Biosensors
Mendoza E., Borowiak-Palen E., Sharpe K., de Silva S.G.M., Mureau N., Silva S.R.P, University of Surrey, UK
Carbon Nanotubes have unique properties such as chemical stability, good mechanical properties and high surface area that makes them ideal for the design of sensors. In this study, we present the use of Carbon Nanotube [...]
Electrochemical Detection of Single-Nucleotide Mismatches: Application of M-DNA
Abu-Irhayem E., Long Y-T., Sutherland T., Li C-Z, Kraatz H-B, Lee J.S., University of Saskatchewan, CA
The detection of a single-nucleotide mismatch in a 20 base pair duplex DNA, using Electrochemical Impedance Spectroscopy, is presented. Mismatched DNA monolayers on Au electrodes were studied as normal duplex DNA (B-DNA) and after conversion [...]
A Novel Electrochemical Nitric Oxide Sensor: Aligned RuO2 Nanowires Deposited on Pt filament
We fabricated a novel electrochemical NO sensor by depositing aligned ruthenium oxide (RuO2) nanowires on a fine Pt filament. This device exhibited a high current density and a low detection limit for NO in aqueous [...]
Effect of Length of Biotin Modified n-Alkanethiolates on the Formation of a Self-Assembled Streptavidin-Biotin Monolayer on a Gold Surface
The molecular recognition between streptavidin and biotin makes the streptavidin-biotin system very useful in a wide range of biotechnological applications. Currently, biotin-modified n-alkanethiolates have been extensively studied to create a wide variety of self-assembled monolayers [...]
Enhancement of E6 Protein Binding on Binding-Orientation-Sensitive Mixed SAMs Molecules
Tseng F-G, Tseng Y.T., Hwang J.K., Hung S.W., Chang J.M., Huang H.M., Huang T.W., Tseng F-G, Tseng Y.T., Huang H.M., Huang T.W., Chieng C-C, National Tsing Hua University, TW
Orientation selectivity and binding enhancement with E6 protein are demonstrated by nano-structured mixed-SAMs systems of different chain lengths. Mixtures of n-alkinethiol SAMs of different chain lengths: 1-Decanethiol (C9) and 1-Hexanethiol (C5) are the examples in [...]
Purification of PCR-Inhibitory Components by Cellulose Acetate Membranes
Mohamed H., Szarowski D.H., Lepak L.A., Spencer M.G., Martin D.L., Caggana M., Turner J.N., New York State Department of Health, US
Conventional methods for extracting DNA from blood results in the presence of heme, which is an inhibitory factor decreasing PCR sensitivity. Current techniques for removing the inhibitors are laborious, costly, and/or sample-specific. We investigated using [...]
Micropatterned Laminin-1 Matrices
Sgarbi N., Pisignano D., Di Benedetto F., Aloisi A., Nicolardi G., Cingolani R., Rinaldi R., Leece University, IT
We have obtained self-assembled laminin-1 networks on glass, realized by one-step microcontact printing lithography, without any preliminary functionalization of the substrates. We investigated the realized patterns by indirect immunofluorescence and atomic force microscopy (AFM), in [...]
Temperature Uniformity and DNA Amplification Efficiency in Micromachined Glass PCR Chip
We have fabricated glass-based micromachined DNA polymerase chain reaction (PCR) chips with different heater patterns. Two pieces of Pyrex 7740 glass are thermally bonded together to form the PCR reaction channel. The PCR channel was [...]
Modeling Particle Hydrodynamic Transport in an Idealized Nanoscale Bio-Motor
In the cell, hydrodynamic characteristics of the nuclear pore complex (NPC) are characterized by Stokesian liquid flow dynamics and high relative particle diffusion rates. As such, the hydrodynamics model of the NPC will be based [...]
Utilizing Nanoscale Patterned Proteins to Create Neuronal Cells Circuits
Wang W., Chun C-J, Gregory C., Narayanan S., Poeta M., Hickman J.J., University of Centra Florida, US
Micro-patterning of neuronal cells in vitro is a critical step for studies in the fundamental biology of neuron-neuron and neuron-surface interactions. The culturing of the neuronal cells on patterned self-assembled monolayers (SAMs), in some cases, [...]
CNTs-Insulator- Semiconductor System for Chemical and Biological Sensor Applications
Cheon J-H, Lin Q., Kong B-D, Kang D.W., Kim L., Suh J.S., Park Y.J., Min H.S., Seoul National University, KR
In this paper, an Electrolyte-CNTs-Insulator-Semiconductor capacitor has been prepared due to compatibility with CMOS technology. To prepare this capacitor, we used the bonding technique to combine the MWNT array.It shows that the surface potential modulation [...]
Microchannel Immunoassays with Electrochemical Detection in Devices Constructed from Low Temperature Co-fired Ceramic and Screen Printed Gold
We report the first self-contained electrochemical enzyme linked immunosorbent assay in microchannel devices constructed from low temperature co-fired ceramic (LTCC) with screen-printed gold for fast and sensitive assays. No one has previously reported a device [...]
Rapid DNA Sequence Identification based on Electrostatic Interactions with Unmodified Gold Nanoparticles
Sequence-specific detection of DNA play more and more important role in clinical diagnosis and molecular biology research. Most assays identify specific sequence through hybridization of an immobilized probe to the target analyte after the latter [...]
Label Free Colorimetric Detection of Single Nucleotide Polymorphisms in Pcr Amplified Genomic DNA
Analysis of DNA is increasingly important in clinical diagnosis, pathology and genetics1, 2. Single nucleotide polymorphisms (SNPs) in genomic DNA are known to be responsible for a number of hereditary conditions3 and cancers4. Nearly all [...]
A Piezoelectric Micro-Cantilever Bio-Sensor Using the Mass-Micro-Balancing Technique with Self-Excitation
A new bio-sensor was developed in order to use in a LOC(Lab-On-a-Chip). The sensor detects the change in the resonance frequency of a micro cantilever with a piezoelectric film(PZT). This is the so-called mass micro-balancing [...]
Electrical Detection of DNA Hybridization using Adjacent Impedance Probing (AIP) Method
Sensitivity and selectivity are two of the most challenging criteria for the development of DNA biosensor devices. These biosensor devices have attracted interest for the rapid identification of pathogens in humans, animals, and plants, for [...]
Controlling Colorimetric Reversibility of Nanostructured Polydiacetylenes
Lee C.H., Lee J.S., Lee C.H., Lee J.S., Park H-W., Lee C.H., Lee J.S., Ahn D.J., Kim J-M, Hanyang University, KR
Polydiacetylene liposome has specific color change of blue to red upon various kinds of stimuli. Stimuli can be a antigen-antybody, thermal stimuli and Ph change and even physical contact. From these charecteristics polydiacetylene used a [...]
A Novel Class of Sensors for System Integrative Concepts in Biotechnological Applications
Schober A., Kittler G., Kittler M., Buchheim C., Majdeddin A., Cimalla V., Fischer M., Yanev V., Himmerlich M., Krischok S., Schaefer J.A., Romanus H., Sändig T., Burgold J., Weise F., Wurmus H., Drüe K.H., Hintz M., Thust H., Gebinoga M., Kittler G., Kittler M., Spitznas A., Gottwald E., Weibezahn K-F., Wegener D., Schwienhorst A., Ambacher O., Technical University of Ilmenau, DE
The application of an system integrative approach based on hetereostructure sensors (group III nitrides) for the examination of differnt biological systems is shown and discussed.
Use of Magnetoresistive Biochips for Monitoring of Pathogenesis Microorganisms in Water through Bioprobes: Oligonucleotides and Antibodies
Martins V.C.B., Fonseca L.P., Ferreira H.A., Graham D.L., Freitas P.P., Cabral J.M.S., Instituto Superior Técnico, PT
Magnetoresistive biochips have been integrated with success in a microtechnology engineered set-up for the development of a portable analytical device, characterized by low-cost with high sensitivity and selectivity of immunodetection and biomolecular methods [1, 2]. [...]
Whole Cell Biosensors For (Eco)Toxicity Screening: Alternatives For The Future
As a response to the emerging environmental threats, the European Parliament introduced tough new rules for the testing and approval of thousands of chemicals. Questions are raised towards the practicality of the new legislation as [...]
Piezoelectric Microcantilevers of Nanoscale Thickness for Detection of Cells
This research project explores the employment of piezoelectric material as the sensing element in microcantilever beams fabricated to function as resonant mass sensors. The cantilever beam would be a very thin double-bridged structure (50 nm) [...]
A Fiber-Optic Biosensor Based on Monooxygenases and Sol–Gel Entrapped Fluoresceinamine for Trichloroethene and Tetrachloroethene
Tetrachloroethene (a.k.a. perchloroethene, PCE) and trichloroethene (TCE) are the most frequently detected groundwater contaminants which are suspected to cause cancer in humans. Monitoring of these carcinogens suffers from high costs and inaccuracy caused by cumbersome [...]
Journal: TechConnect Briefs
Volume: 1, Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Published: May 8, 2005
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topics: Biomaterials, Chemical, Physical & Bio-Sensors
ISBN: 0-9767985-0-6