Authors: A. Llobera, R. Wilke and S. Büttgenbach
Affilation: Institut für Mikrotechnik, Germany
Pages: 390 - 393
Keywords: micro total analysis systems, laser induced fluorescence, optical detection
A new robust and compact system, which comprises a PDMS-based hollow Abbe prism and microlenses, together with self-alignment channels for optical fibre positioning, is presented. This optical detection method has two different regimes for signal transduction, namely absorption and refractive index shift. µM concentrations of fluorescein are detected with this system using standard equipment (SLED and photodetector). The results clearly show the regions in which each physical effect is significant.