Bio Nano Analysis and Characterization Chapter 2

Effect of Substratum Morphology on Cell Behavior

Authors: W-T Su, I-M Chu, J-Y Yang and C-D Lin

Affilation: National Nano Device Laboratories, Taiwan

Pages: 74 - 76

Keywords: contact guidance, stromal, cytoskeletal, plasma etching, microfabrication

Abstract:
Contact guidance induced by the surface topography of the substrata influences the direction of cells with artificial material. Mouse bone marrow stromal fibroblast was examined on grooved substrata of varying dimension (0.3£gm~1.2£gm groove width, 0.5£gm depth and 0.5£gm ridge). It was found that the repeat space had an effect in determining cell alignment. Measurement of cell alignment and examination by scanning electron microscopy showed the fibroblast interacted grooved substrata in monomodal responses and alignment to the direction of the grating.

ISBN: 0-9728422-7-6
Pages: 521
Hardcopy: $79.95