2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Landscape phage probes for PC3 prostate carcinoma cells

P.K. Jayanna, P. Deinnocentes, R.C. Bird, V.A. Petrenko
Auburn University, US

landscape phage, prostate cancer, drug delivery, targeting

Cancer chemotherapy is complicated by a lack of selective drug targets. The use of liposomes as vectors for drug delivery has provided a route to selective accumulation of nanoparticles in the tumor interstitium due to EPR effect. To further enhance tumor chemotoxicity of liposomal drugs, they have to be precisely targeted to tumor receptors using tumor-specific probes. Herein, we describe the use of landscape phage libraries for deriving specific phage probes for PC3 prostate tumor cells. In our selection protocol, we used an extensive depletion regimen to rid the parent library of non-specific binders followed by four rounds of selection for each library. Phage probes bound to the surface were collected by elution with acid buffer (Eluate fraction) whereas the phage probes internalizing into the cells were collected followed by lysis of cells (Lysate fraction). The selected clones will be tested for their target binding efficiency in cell binding assays. Positive clones will be further tested for selectivity to target cells by comparing relative binding efficiencies to human skin fibroblasts, hepatocytes and hepatocarcinoma cells. The probes identified by these protocols will be studied as potential targeting ligands for liposomes and other nano-particulate drug vehicles.

Nanotech 2008 Conference Program Abstract