Nanotechnology Conference and Trade Show - Nanotech 2006
> Program > Technical Conferences > Business & Development > Nano Impact Workshop > Nanotech Job Fair > Expo
Index of Authors
Index of Keywords
Confirmed Speakers
Conferences & Symposia

Conference Proceedings

Conference Technical Proceedings

Cell and Particle Concentration on the Chip

D. Irimia and M. Toner
Massachusetts General Hospital, Harvard Medical School, US

valve, cell suspension, large particles

Lab-on-a-chip devices for cell processing and analysis are increasingly sophisticated and targeting a wider spectrum of scientific research and clinical diagnostic applications. Despite recent advances however, many sample preparation steps, common for macro-scale techniques, have not yet been implemented in microfluidic systems. One example is centrifugation, which is commonly employed for increasing the concentration the chip. Here we present a new microfluidic design, physically implemented using standard soft lithographic techniques, which enables the handling of suspensions of eukaryotic cells and three orders of magnitude increase of the cell concentration. In a significant departure from existent elastomeric microscale valves, our approach allows the integration of multiple structures on the same membrane, and the execution of complex sequential procedures using a limited number of control steps. In our design, a mobile sieving barrier in closed position could trap cells mechanically, and could be subsequently opened to allow the trapped cells to pass into an outlet channel. At the same time, the suspension fluid passing through the 3 x 10 µm openings in the sieve, was directed not into the outlet channel but instead was guided into a drain channel and removed from the cell suspension, by a second barrier.

Back to Program

Sessions Sunday Monday Tuesday Wednesday Thursday Authors

Nanotech 2006 Conference Program Abstract

Nanotechnology Conference | Terms of use | Privacy policy | Contact | NSTI Home
Program | Technical Conferences | Business & Development | Nano Impact Workshop | Nanotech Job Fair | Expo |
Nanotech 2006 Home | Press Room | Venue | Subscribe | Site Map
Names, and logos of other organizations are the property of those organizations and not of NSTI.
This event is not open to the general public and NSTI reserves the right to refuse admission and participation to any individual.