Authors: E.T. Chen, J. Thornton, C. Ngatchou, S-H. Duh, P.T. Kissinger
Affilation: Advanced Biomimetic Sensors, Inc., United States
Pages: 107 - 110
Keywords: nanobiomimetic sensing, biomimetic pyruvate dehydrogenase kinase (PDK) 3 and lipoyl binding pocket, hydrophobic interaction, selectivity
Diseases such as cancer, diabetes mellitus, and brain injury can be caused by lack of conformational change in the pyruvate dehydrogenase complex (PDC). The known “ATP Lid” for the function of PDC is comprised by the complex of L2 forming a flat bridge with PDK3 nanopore structured subunit. We report the new nanobiomimetic PDC sensors having the ability to detect single brain cancer cell of SNB-19, while rejecting breast cancer cells of MDA-MB-231 from 1 to 100 cell/mL without using antibody and without labeling. Three gold sensors were developed and studied by cyclic voltammetry (CV) and chronoamperometric (CA) methods. Membrane morphology was characterized by an AFM method. Selectivity studies comparing Sensor 1 (with a vertical bridge structure with nanopore breathing core) with Sensor 2 (with a flat bridge structure and nanopore) were carried out for 2 types of cancer cells. Sensor 3 with a nanopore without a bridge also considered. Sensor #2 offered orders of magnitudes higher signal intensity for the brain cancer cell vs. Sensor 1. Sensor #2 is superior in selectivity for 1 cell/mL brain cancer cell over breast cancer cell.