Nano Science and Technology Institute
Nanotech 2012 Vol. 3
Nanotech 2012 Vol. 3
Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Chapter 2: Bio Nano Materials

Unravelling adsorption and alignment of amyloid fibrils at interfaces by probe particle tracking

Authors:L. Isa, J.M. Jung, R. Mezzenga, S Jordens
Affilation:ETH Zurich, CH
Pages:72 - 75
Keywords:amyloid protein fibrils, self-assembly, liquid interfaces, particle tracking, isotropic/nematic
Abstract:We report the first direct, non-invasive experimental evidence of a 2D isotropic–nematic transition for highly anisotropic nanoparticles at liquid–liquid interfaces by using passive fluorescent particle tracking. In order to illustrate the potential of this approach on systems of high real practical and biological relevance, we select as a model anisotropic nanoparticles b-lactoglobulin amyloid fibrils of varying aspect ratios. Upon nanoparticle adsorption at the interface, we follow, in real time and as a function of fibril length and bulk concentration, the development of a 2D nematic phase by studying the anisotropy in probe particle traces. We furthermore demonstrate the long-range nature of the nematic phase by calculating order parameters for the traces as high as 0.8 over 10^2 to 10^3 mm^2 areas. The presented route is independent of the system investigated, and thus these findings open a new, general strategy for the experimental assessment of 2D structural changes at anisotropic fluid–fluid interfaces.
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