Authors: D. Patel, G. Zhang, X. Sun, A.M. Gobin, R.S. Keynton
Affilation: University of Louisville, United States
Pages: 17 - 20
Keywords: gold nanoshells, biomarkers, detection
The focus of this project is to improve the detection of antigen/biomarkers/viral vectors using gold nanoshells (GNS). The assay used in this project is based on aggregation of GNS in presence of antigen or viral vectors. GNS are coated with a Protein-G conjugated to thiol–poly(ethylene) glycol–amine and Goat anti-rabbit antibody. Our data shows that fully assembled nanoparticles in presence of antigen, rabbit IgG , allow detection at concentrations in the ng/ml to pg/ml. The lower detection limit of this improved assay was 0.5 pg/mL within 10 mins. This shows a 3 order of magnitude improvement over that previously reported.