Authors: J. Podczeviensky, J. McDowell, L. Levine
Affilation: ALine, Inc., United States
Pages: 432 - 435
Keywords: microfluidics, point of care, lab-on-a-chip
Microfluidics are critical to the development of high sensitivity, multiplexed, and quantitative point-of-care diagnostic products that promise to improve the quality of healthcare while lowering costs. Fundamental to a shift from qualitative screening tests to quantitative measurements near the patient is the need for a robust fluidic system that is tolerant of the method of manufacture, has robust interconnects to the instrument, with optimized actuation routines. In this paper, we will discuss the variability in the performance of on-board pumps and valves under a set of test conditions that mimic a real assay protocol using an instrument test bed with an engineered instrument interface. We will discuss the impact of air bubbles and alignment tolerances on the variability in fluid metering and mixing and offer suggestions for improving performance.