Authors: M.-H. Chang, F.-C. Ding, W.-Y. Ju, P.S. Lai
Affilation: National Chung Hsing University, Taiwan
Pages: 287 - 290
Keywords: antimicrobial, wound dressing, photocurable
To develop an appropriate approach that can accelerate the wound healing rate or prevent other microorganisms contamination is necessary for wound healing. In this study, the combination of antimicrobial activity and photodynamic antimicrobial chemotherapy of photocurable wound dressing for wound healing were evaluated in vitro and in vivo. The wound dressing with photocurable gelatin /chitosan/methylene blue mixed solution could be formed fast after UV light irradiation. The antimicrobial activity and bacteriostasis efficiency of this photocurable composite were evaluated by colony assay and disc agar diffusion method, respectively. The best composition of photocurable composite on bacteriostasis efficiency (around 13 mm) was chosen to further study the wound-healing in microorganism (Staphylococcus aureus) infected wounds. After UV light irradiation, film-liked wound dressing was formed to protect wounds from other microorganism contaminations. In contrast, untreated group needed three days to reach the similar scab level. Moreover, a better appearance of wounds on the dorsal of mice could be observed on photocruable wound dressing group after 13 days. These results suggested that the photocurable composite may not only exhibit good antimicrobial activity but show the potential to be an ideal wound dressing for wound healing.