Authors: J.C.K. Lai, Y. Wang, W. Gao, H. Gu, S.W. Leung
Affilation: Idaho State University, United States
Pages: 233 - 235
Keywords: biosensor, nanoparticle, peroxide, nitrite, self-assembly
For the third generation of biosensor that electrode is coupled directly with enzymes, the response time for detection is rapid but stability of the measurements and the coupled enzymes is a concern. In our research group, we are developing biosensors that are based on electrodes modified with sol-gel nanoparticles that bind with different enzymes for our detection targets. In this report, we studied the performance of Au, Ag, and glassy carbon electrode that were modified with nano Au particles. The electrodes were then coated with hemoglobin by self-assembly technique. These electrodes were then used to detect nitrite and peroxide for their performance comparisons. All three electrodes were fast and responsive for the detection of nitrite, with response time within seconds and concentrations ranged from mM to below mM. The Au electrode was the most stable among the three electrodes after multiple cycles of measurements with the cyclic voltammographic technique, the Ag electrode was the least stable, and the glassy carbon electrode behaved similarly with that of the Au electrode. Results of the measurements with peroxide by the three electrodes were compatible with the performances of nitrite measurements.