Authors: A.J. Kell, C. Paquet, B. Simard
Affilation: National Research Council, Canada
Pages: 210 - 213
Keywords: superparamagnetic nanoparticle, bacteria, luminescent, vancomycin, antibody, quantum dot
Infectious diseases and bioterrorism are of tremendous global concern, and our group is interested in developing superparamagnetic nanoparticle platforms that can aid in the magnetic extraction/concentration and detection of harmful pathogens. This has led to the development of both small molecule probe-modified nanoparticles for the affinity magnetic capture of multiple species of Gram-positive and Gram-negative bacteria and single-domain antibody-modified nanoparticles for the selective capture of a single bacteria species, for example, S. aureus. We have demonstrated that the size of nanoparticles supporting the probe molecules can affect both the ability for these molecules to effectively interact with receptors on the bacteria surfaces and the speed of pathogen confinement. In parallel we have been developing nanoparticle detection platforms that utilize hybrid magnetic/luminescent nanoparticles comprised of quantum dots or fluorescent dyes and superparamagnetic nanoparticles. As such, we will briefly discuss how these hybrid nanoparticles can be exploited for simultaneous magnetic confinement and optical detection of multiple pathogens from a single sample. Together the results of this presentation will highlight the power of combining hybrid nanoparticles and developing methods for the controlled surface modification for the fast and efficient isolation and detection of pathogenic species from biological samples.