Authors: X. Liu, Q. Dai, J. Coutts, L. Austin, G. Knowles, Q. Huo
Affilation: University of Central Florida, United States
Pages: 256 - 258
Keywords: gold nanoparticle, bioconjugate, molecular imaging, biomarker
Gold nanoparticles and colloids have been used for a long time in biomolecular imaging and detection. With further understanding on many of the electrical and optical properties of gold nanoparticles revealed recently, the application of gold nanoparticles in this field has been extended enormously. The unique properties of gold nanoparticles include large optical absorption and light scattering cross section, surface plasmon resonance, surface-enhanced Raman scattering, size and organization-dependent optical property change, catalytic effect, etc. These properties make gold nanoparticles especially attractive for optical imaging and detection. In this talk, we will present some of our most recent work on the synthesis, surface functional group modification, and bioconjugation chemistry of gold nanoparticles. We explored a number of methods to increase the stability of gold nanoparticles and the bioconjugation efficiency of the nanoparticles with antibodies and DNA probes. A solid phase modification technique will be introduced for controlled chemical functionalization of gold nanoparticles. The structural integrity and biological activity of the nanoparticle bioconjugates will be discussed. The surface chemistry of the nanoparticles plays a critical role in retaining the biological function of the molecules. The applications of these surface-modified gold nanoparticles for cancer biomarker detection and cell imaging will be presented in the talk.