Authors: A. Jaiswal, S. Smoukov, M. Poggi, B. Grzybowski
Affilation: Q-Sense, Inc., United States
Pages: 855 - 858
Keywords: QCM-D, nanoparticles, biointerface, dissipation, polymer
There has been an increasing demand for analytical tools that can quantify the interaction and/or reaction of nano-scale particles, polymers as well as bio-molecules, with various surfaces. In-situ monitoring of structural and dynamic changes of these materials is of critical importance. With an understanding of the behavior of these molecules at nano-scale, conditions for desired results can be optimized. Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D), a nanomechanical acoustic-based analytical technique, provides in situ, real-time characterization of the interactions of various polymeric and bio-materials at variety of interfaces. With QCM-D, simultaneous measurement of resonance frequency change ( F) and energy dissipation change( D) is performed by periodically switching off the driving power of oscillation of the sensor crystal and recording the decay of damped oscillation as the adsorption and/or structural changes takes place at sensor crystal surface (Figure 1). While change in frequency provides information about mass changes, changes in dissipation (D) provide structural information about the viscoelastic properties of adsorbed films in real time. This presentation will be focused on application of the QCM-D technique in real-time characterization of nanoparticle adsorption (Figure 2), and interaction of various biomolecules such as proteins, DNA, vesicles, cells and viruses with other biological materials, polymers or inorganic surfaces.