Authors: K. Borchers, A. Weber, S. Genov, I. Wirth, A. Grzesiak, G.E.M. Tovar
Affilation: Fraunhofer Institute for Interfacial Engineering and Biotechnology, Germany
Pages: 428 - 430
Keywords: layer-by-layer, ring-pin, nanoparticle layers, molecular recognition
Nanoparticles display a very large surface-area. Therefore, coatings of functional nanoparticles adsorbed to a solid substrate yield an enlarged interface per footprint-area which is highly appreciated for probe-immobilization in bio-chip technology [1,2]. Silica nanospheres, equipped with an organic shell, can be used to couple specific capture-proteins or DNA-probes by application of state-of-the-art bioconjugate chemistry . Further to spotting of biofunctional nanoparticles in a micro arrayed format , the generation of a covering nanoparticle-coat in a user-defined shape  allows for flexible assay-design and for fabrication of large bioconjugative interfaces on both, glass and polymer substrates. In view of the advantages that have been experienced in the past decade using biochip- based screening-approaches, biochip-production should be made a cheap, flexible and reproducible process. In order to set up an automated production-line for nanoparticle-based bio-chips we are developing an ink-jet printing process that is compatible with the treatment of biomolecules and that allows for high flexibility as well as accuracy in nanoparticle deposition. We will present results concerning substrate-activation, ink-preparation, the print-performance treating ink-formulations containing (bio)functional nanoparticles and the maintenance of biological functions during the ink-jet process. References  Hauser, N., Tovar, G.E.M.; Biospektrum, 9, 6, 2003 710  Tovar, G. E. M., Weber A.; Ency. Nanosci. Nanotechnol. 1 2004 277-286.  Schiestel, T., Brunner, H., Tovar, G.E.M.; J. Nanosci. Nanotechn. 4 2004 504.  Borchers, K., Weber, A., Brunner H., Tovar, G. E. M.; Anal. Bioanal. Chem. 383 2005 738.  Weber, A ., Knecht, S., Brunner, H., G. E. M. Tovar; Engineering in Life Sciences 4 2004 93-97.