Authors: N.O. Chahine, N.M. Collette, H. Thompson, G.G. Loots
Affilation: Lawrence Livermore National Lab, United States
Pages: 125 - 128
Keywords: tissue regeneration, nanocomposites, cytotoxicity
In this study, we assess the long-term biocompatibility of CNTs for chondrocyte growth. The effect of CNTs on chondrocyte viability and biochemical deposition are examined in 2D cultures, and in 3D composite materials consisting of hydrogels + CNTs. The exposure of chondrocytes to CNTs was carried out up to 14 days in 2D culture and 21 days in 3D agarose composites. Our results suggest that functionalized CNTs alter the viability and metabolic response of cells over the 2 to 3 week duration, in a dose dependent manner. The results of this study indicate that SWNTs offer a unique potential for cartilage tissue engineering, where future attempts at functionalization with bioactive molecules may provide an ideal substrate for stimulation of cellular growth and repair.