Authors: T. Snabe, M.T. Neves-Petersen, P. Fojan, S. Klitgaard and S.B. Petersen
Affilation: Aalborg University, Denmark
Pages: 398 - 401
Keywords: biosensor, UV, protein, immobilization, nanoarray
Fundamental knowledge on protein structures and the effect of ultraviolet light on these structures has paved the way for the development of a unique light-based immobilization technology that allows oriented protein immobilization onto micrometer sized spots. The methodology is considered to be a strong alternative to the conventional procedures which often include the use of harsh conditions such as strong chemicals and elevated temperatures. The technology behind this immobilization technique - here termed "light assisted immobilization" - is based on the fact that disulphide bridges that are naturally present within the protein structure can be broken as a result of UV-illumination. The free thiol groups (-SH) created upon disruption of a disulphide bridge are very reactive and can be used as linkers for covalent attachment to a surface. The surface can for example be gold or thiol-derivatized silicon, making this technology extremely useful for a large range of application areas, including biosensors.