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 | Nanotech 2004 Vol. 1
Technical Proceedings of the 2004 NSTI Nanotechnology Conference and Trade Show, Volume 1
Chapter 6: Bio Micro Sensors and Systems |
| | Optical Detection of Integrin AvB3 Using a Nanostructured Lipid Bilayer Membrane Biosensor | | Authors: | O. Worsfold and T. Nishiya | | Affilation: | Fujirebio Inc., JP | | Pages: | 217 - 220 | | Keywords: | integrin, optical, bionanosensor, lipid membrane | | Abstract: | An ultra-sensitive optical bionanosensor platform for integrin avb3 loaded human umbilical vein endothelial cells (HUVECs) was fabricated. The bionanosensor design involved the incorporation of an RGD (arginine-glycineaspartate) containing peptide conjugated BODIPY lipid soluble dye (donor) into a substrate supported bilayer lipid membrane (SBLM). The RGD peptide binds with the integrin avb3 via a multimerisation mechanism thus reducing the energy transfer from the donor dye moiety to a second higher excitation wavelength BODIPY lipid soluble dye (acceptor) moiety. The reduction in energy transfer is visualised by an increase in the fluorescence intensity of the donor and a decrease in the fluorescence intensity of the acceptor. The surface chemistry of the substrate has a significant effect on the fluidity of the lipid bilayer (measured by Fluorescence Recovery after Photobleaching (FRAP)) and on the subsequent biosensing response. Using this novel bionanosensor architecture a sensitivity of 25 HUVEC cells/ml was recorded. | | ISBN: | 0-9728422-7-6 |
| Pages: | 521 |
| Hardcopy: | $150.00 |
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