Advances in Biological Sensing and Manipulation by DPN and SERRS
University of Strathclyde, UK
Keywords: DNA, Detection, Molecular, Metrology
Abstract:The greatest advances in modern science have come about through the crossing of traditional boundaries to yield multidisciplinary research teams capable of tackling larger scientific problems that require a range of different expertise and skills. The Centre for Molecular Nanometrology at the University of Strathclyde in Scotland has been created in an effort to try and harness some of this approach in tackling modern problems involving measurement on a nanoscale. Our traditional interests have been in using a combination of functionalized nanoparticles with optical spectroscopy to allow us to use surface enhanced resonance Raman scattering (SERRS) to detect biological sequences with excellent sensitivity and in a multiplexed fashion. More recently we have become interested in the use of structured metal surfaces to provide the surface enhancement component of the SERRS and coupled with this dip pen nanolithography (DPN) to give us control over the placement of specific molecules on these surfaces. This presentation will focus on our use of specific chemistry which is compatible with the DPN approach for functionalizing metal and glass surfaces with a view to doing combined optical measurements of these highly structured surfaces. Examples include, DNA detection by use of DPN to ‘write’ specific capture sequences onto a structured metal surface and subsequent detection by SERRS, writing of proteins as nanoarrays and subsequent analysis of interactions by SERRS and the control of differentiation of stem cells by nanopatterned surfaces produced by DPN. We look at this as a top-down/bottom-up assembly process that yields information on biological systems and offers excellent opportunities for new avenues of scientific exploration.